Iranian Journal of Parasitology 2016. 11(3):290-295.

Leishmania major: Genetic Profiles of the Parasites Isolated from Chabahar, Southeastern Iran by PPIP-PCR


Background: Leishmaniasis is important vector-borne parasitic disease worldwide, caused by the genus Leishmania. The objective of the current study was to identify genetic polymorphism in L. major, one of the species causing cutaneous leishmaniasis (CL), isolated from southeastern Iran, using Permissively Primed Intergenic Polymorphic-Polymerase Chain Reaction (PPIP-PCR) method.

Methods: Overall, 340 patients with suspected CL were examined. They referred to the Central Laboratory in Chabahar, Iran during Apr 2013 to Feb 2014. Microscopic examination of Giemsa-stained slides from lesions as well as aspirates cultured in Novy- Mac Neal-Nicolle (NNN) Media was employed in order to diagnose CL in these patients. Our analyses detected 86 suspected subjects as having CL from which 35 isolates were cultured successfully. PPIP-PCR method was performed on extracted genomic DNA from selected isolates in order to determine the genetic polymorphism among L. major isolates.

Results: The electrophoresis patterns demonstrated two genetic profiles including A or A1 patterns between all samples tested. Frequency of A and A1 sub-types were 33 (94.3%) and two (5.7%), respectively.

Conclusion: Both host and parasite factors may contribute to the clinical profile of human leishmaniasis in the endemic foci of the disease. Here we showed that genetic variations pertaining to the Leishmania parasites might determine, in part, the clinical outcomes of human leishmaniasis. 


PPIP-PCR; Leishmania major; Genetic polymorphism; Iran

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WHO. Control of the Leishmaniasis. Report of a meeting of the WHO expert committee on the control of Leishmaniasis; Geneva. Important document summarizing the epidemiology and latest diagnostic and treatment recommendations for all forms of leishmaniasis, per region. 2010.

Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M. Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 2012;7(5):e35671.

Sharifi I, Ardehali S, Motazedian H, Aflatoonian MR, Fekri AR. Identification and characterization of Leishmania isolates in school children in Bam, southeastern Iran. Iran J Med Sci. 1997;22(3,4):82-8.

Eisenberger CL, Jaffe CL. Leishmania: Identification of old world species using a Permissively Primed Intergenic Polymorphic - Polymerase Chain Reaction. Exp Parasitol. 1999;91:70-7.

Fazaeli A, Fouladi B, Hashemi-Shahri SM and Sharifi I. Clinical Features of Cutaneous Leishmaniasis and Direct PCR-Based Identification of Parasite Species in A New Focus in Southeast of Iran. Iran J Public Health. 2008;37(3):44-51.

Kassiri H, Naddaf SR, Javadian EA, Mohebali M. First Report on Isolation and Characterization of Leishmania major from Meriones hurrianae (Rodentia: Gerbillidae) of A Rural Cutaneous leishmaniasis Focus in South-Eastern Iran. Iran Red Crescent Med J. 2013;15(9):789-93.

Veland N, Boggild AK, Valencia C, Valencia BM, Llanos-Cuentas A, Van der Auwera G, Dujardin JC, Arevalo J. Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach. J Clin Microbiol. 2012;50:495–8.

Parhizkari M, Motazedian MH, Asqari Q, Mehrabani D. The PCR-based detection of Leishmania major in Mus musculus and other rodents caught in southern Iran: A guide to sample selection. Ann Trop Med Parasitol. 2011;105:319–23.

Carvalho EM, Donelson JE, Wilson ME. Serial quantitative PCR assay for detection, species discrimination, and quantification of Leishmania spp. in human samples. J Clin Microbiol. 2011;47:3892–904.

Schönian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis. 2003;47:349–58.

Jaffe CL, Baneth G, Abdeen ZA, Schlein Y, Warburg A. Leishmaniasis in Israel and the Palestinian Authority. Trends Parasitol. 2004;20:328–32.

Dabirzadeh M, Mirmohammad Sadeghi H, Baghaie M, Hejazi H. Genetic Polymorphism of Leishmania major in Two Hyper Endemic Regions of Iran Revealed by PPIP-PCR and ITS-RFLP. Arch Iran Med. 2012;15(3):151-6.

Layegh P, Moghiman T, Ahmadian Hoseini SA. Children and cutaneous leishmaniasis: a clinical report and review. J Infect Dev Ctries. 2013;7(8):614-7.

Talari SA, Talaei R, Shajari G, Vakili Z, Taghaviardakani A. Childhood cutaneous leishmaniasis: report of 117 cases from Iran. Korean J Parasitol 2006;44:355-60.

Tibayrenc M. Population genetics of parasitic protozoa and other microorganisms. Adv Parasitol. 1995;36:47 – 115.

Evans D. Handbook on Isolation, Characterization and Cry Preservation of Leishmania UNDP/World Bank/World Health Organization. Geneva, Switzerland. 1989.

Kelly JM, Law JM, Chapman CJ, Van Eys GJ, Evans DA. Evidence of genetic recombination in Leishmania. Mol Biochem Parasitol. 1991;46:253 – 63.


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