Exploration of Sarcoptes scabiei Antigenic Protein Which Play Roles in Scabies Pathogenesis in Goats and Rabbits

  • Nunuk Dyah Retno LASTUTI Dept. of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Poedji HASTUTIEK Dept. of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Lucia Tri SUWANTI Dept. of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
  • Dony CHRISMANTO Dept. of Animal Health, Faculty of Vocational, Universitas Airlangga, Surabaya, Indonesia
Keywords: Sarcoptes scabiei var. caprae, Sarcoptes scabiei var.cuniculi, Mites, Antigenic proteins

Abstract

Background: Scabies or mange is an infectious skin disease caused by the mite Sarcoptes scabiei. This skin disease affects various livestock such as goats, sheep, swine, cattle, other animals like dogs, cats, wild animals and also affect human. This research aimed to explore the protein in mites S. scabiei which has antigenic character and play roles in scabies pathogenesis in goats and rabbits. Methods: S.scabiei mites were isolated from goats and rabbits, and characterized using SDS-PAGE. In addition the protein was also analysed using Western Blot assay. The isolation and identification were carried out in 2015 at the Parasitology Laboratory of Veterinary Medicine Faculty, Universitas Airlangga, Surabaya, Indonesia. Results: The identification results using SDS-PAGE of mites S. scabiei var. caprae expressed 12 protein bands between 26,7 kDa and 205,8 kDa, continued by Western Blot showed 3 protein bands, after being reacted with blood serum from scabies infected goat, it could be identified antigenic protein with molecule weight 205.8 kDa, 57.3 kDa, and 43 kDa. While protein in mites S. scabiei var. cuniculi identified 9 protein bands between 24 kDa and 75 kDa by SDS-PAGE, and the Western Blot assay identified antigenic protein with molecule weight 62 kDa and 51 kDa. Conclusion: The antigenic protein of S. scabiei var. caprae and S. scabiei var. cuniculi showed that they are probably involved in the scabies pathogenesis in goats and rabbits.

Author Biographies

Nunuk Dyah Retno LASTUTI, Dept. of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
Departement of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga
Poedji HASTUTIEK, Dept. of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
Departement of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga
Lucia Tri SUWANTI, Dept. of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
Departement of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga
Dony CHRISMANTO, Dept. of Animal Health, Faculty of Vocational, Universitas Airlangga, Surabaya, Indonesia
Departement of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga

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Published
2018-09-24
How to Cite
1.
LASTUTI NDR, HASTUTIEK P, SUWANTI LT, CHRISMANTO D. Exploration of Sarcoptes scabiei Antigenic Protein Which Play Roles in Scabies Pathogenesis in Goats and Rabbits. IJPA. 13(3):466-72.
Section
Original Article(s)